SEDEX FP — Powerful, Reliable LT‑ELSD Detection for Purification Workflows

High‑Performance LT‑ELSD for Preparative LC, Flash & SFC Purification

The SEDEX FP delivers robustness, sensitivity and operational simplicity for purification workflows.
Powered by SEDERE’s proven Low‑Temperature Evaporative Light‑Scattering Detection (LT‑ELSD)technology, it provides strong, stable and universal mass‑based detection for compounds that UV, RI or MS often fail to monitor effectively during preparative or high‑load chromatographic steps.

Its compact 10‑inch footprint, modern optical architecture and optimized electronics make it a natural fit for preparative HPLC, preparative SFC and Flash Chromatography systems.
Complete SOP documentation enables fast, GLP‑compliant validation and seamless integration into R&D, pilot‑scale or production environments.

The SEDEX FP can be controlled either locally or through chromatography software, with a remote automated shutdown mode that preserves instrument lifetime and reduces running costs.

Mass‑Based, Homogeneous Response for Accurate Purification Monitoring

A major advantage of LT‑ELSD detection—and a defining strength of the SEDEX FP—is its ability to deliver a highly homogeneous mass‑based response, regardless of the chemical nature of the analytes.

This means:

• Peak size on the chromatogram correlates directly with the actual amount of compound,
• Compounds with very different structures produce responses proportional to their mass in the sample, not to their properties,
• Method development and fraction triggering become significantly more intuitive.

In contrast, UV detection depends heavily on the molar extinction coefficient (ε) of each analyte, as defined by the Beer–Lambert law. Two compounds present in identical quantities can produce completely different UV peak sizes simply because their ε values differ — making purification monitoring less reliable, especially for mixtures of structurally diverse natural products, polymers, lipids, terpenes, peptides, etc.

Because LT‑ELSD measures light scattering from dried particles rather than absorbance, the SEDEX FP offers a more representative and more intuitive tracking of purification profiles.
Conclusion: for purification processes, LT‑ELSD delivers clearer, more truthful chromatographic feedback than UV detection, enabling better fraction collection decisions and reducing the risk of product loss or cross‑contamination.

Key Advantages of the SEDEX FP

✔ True Low‑Temperature Optical Detection

Superior sensitivity while preserving semi‑volatile and thermally fragile compounds.

✔ Dedicated Purification‑Optimized Nebulizer

Minimizes band broadening and ensures stable peak shapes during high‑flow purifications.

✔ Broad Flow Rate Compatibility

Handles 100 µL/min to 5 mL/min — ideal for preparative HPLC, Flash and SFC.

✔ SAGA Automatic Gain Control

Prevents off‑scale saturation and maintains optimized detector response throughout purification runs.

✔ Anti‑Clogging Flow Path

Internal geometry designed to reduce contamination and blockage risks from concentrated or particulate‑rich samples.

✔ Full Remote Control

Automatic shutdown of gas, heater, photomultiplier and light source at end of sequence.

✔ Universal Detection

Detects virtually any compound less volatile than the mobile phase — no chromophores required.

 Ideal Applications

The SEDEX FP excels in high‑load purification environments requiring universal, mass‑based detection:

✔ Preparative HPLC

Reliable monitoring of complex mixtures where UV fails due to varying extinction coefficients.

✔ Flash Chromatography

Fast, high‑flow separations benefit from strong, stable optical‑scattering response.

✔ Preparative SFC

Ideal for thermosensitive, semi‑volatile and structurally diverse analytes.

✔ Natural Product & Extract Purification

Lipids, terpenes, surfactants, pigments, alkaloids, carbohydrates, polyphenols, plant extracts, marine extracts…

✔ Fraction Triggering Without Chromophores

Perfect for compounds invisible to UV or displaying inconsistent UV response due to ε variability.

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